Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Sp9

Cell type

Cell type Class
Others
Cell type
Olfactory epithelium
NA
NA

Attributes by original data submitter

Sample

source_name
olfactory bulb
strain
CD1
age
postnatal day 0-2
tissue
olfactory bulb
chip antibody
Sp9 (homemade antibody)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The lysate was sheared into 200- to 1000-bp fragments and Sp9-DNA complex was isolated with specific Sp9 antibody. The yield of ChIP DNA was determined via Quant IT fluorescence assay (Life Technologies) and enrichment efficiencies of ChIP reaction were evaluated by qPCR. Illumina sequencing libraries were generated with NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) by following the manufacturer's manual. The library quality was determined by using Agilent 2100 Bioanalyzer(Agilent), and then, subjected to high-throughput 150 base paired-end sequencing on Illumina Hiseq sequencer according to the manufacturer’s recommended protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
23882224
Reads aligned (%)
71.4
Duplicates removed (%)
17.1
Number of peaks
47752 (qval < 1E-05)

mm9

Number of total reads
23882224
Reads aligned (%)
71.2
Duplicates removed (%)
17.0
Number of peaks
47655 (qval < 1E-05)

Base call quality data from DBCLS SRA